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QSB Seminar Series 291 (12/2/16)

December 2, 2016 - 1:30pm

Flyer File

Steven Brown, University of California, San Diego


In modern models of cell growth and division in Escherichia coli, a parental cell grows and divides asymmetrically to produce two identical daughter cells. However, once a cell pole is formed, no new pole will be formed between the nucleoid and any existing cell pole. This creates a time-related asymmetry after further rounds of division wherein one cell pole has existed for a longer time than the opposite cell pole. We previously reported that the rate of death of old-pole cells increases as the replicative age of the cell increases. To investigate the mechanism of this changing death rate, we characterized the mode of death for thousands of old-pole cells. We find that the change in the rate of cell death is attributable to a change in nucleoid positioning within aging cells. This change in nucleoid positioning is associated with changes in cell septum formation position and cell length which ultimately produces non-growing anucleate cells at the old cell pole. The formation of anucleate cells does not appear to involve obvious nucleoid pathologies, suggesting that these events are not “death” in the sense that genetic information ceases to replicate. Excluding the formation of anucleate cells, the rate of death by other mechanisms is approximately constant with replicative age between 0.001 and 0.002 per cell per generation.


Classroom and Office Building 267

Contact Information

Jing Xu
Assistant Professor
School of Natural Sciences